Coding

Part:BBa_K1674004:Design

Designed by: Roni Cohen   Group: iGEM15_Technion_Israel   (2015-09-15)

zwf - Glucose-6-Phosphate-Dehydrogenase from E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The zwf gene was cloned, after PCR, into pSB1C3. The sequence was validated by sequencing.

Source

Prof. Hanna Engleberg-Kulka from the Hebrew University of Jerusalem gave us a pQE32 plasmid containing the zwf gene.

References

  1. Sang-Jun L.; Young-Mi J.; Hyun-Dong S.; Yong-Hyun L.: Amplification of the NADPH-related genes zwf and gnd for the oddball biosynthesis of PHB in an E.coli transformant harboring a cloned phbCAB operon. Journal of bioscience and bioengineering. 2002. 93. 543-549.
  2. Uwe S.; Fabrizio C.; Sylvia H.; Annik P. Eliane F.: The soluble and membrane-bound transhydrogenases UdhA and PntAB Have divergent function in NADPH metabolism of Escherichia coli.
  3. Susan S. H.; John E. P.; Pedro M. A.; Trevor M. P.; Mitchell L.: Tree-dimensional structure of rat liver 3α-hydroxysteriod/dihydrodiol dehydrogenase: A member of the aldo-keto reductase superfamily. PNAS. 1994.91. 2517-2521.
  4. Fabrizio C.; Tracy A. H.; Sylvia H.; Taotao W.; Thomas S.; Uwe S.: Metabolic flux response to phosphoglucose isomerase knock-out in Escherichia coli and impact of overexpression of the soluble transhydrogenase UdhA. FEMS Microbiology Letters. 2001. 204. 247-252.